Quartz optical fibers for fluorescence measurement are configured in the LOV module at right angles (A). The beads are captured within the flow cell by the vertical optical fiber, the sheath of which is designed to form a 30 μm gap between the fiber and the conduit wall. The optical fiber is held in place by a ferrule and the carrier stream exits by a side channel.
Since fluorescence measurements have to be carried out while the flow cell is shielded from external light, the filling of the flow cell with beads cannot be visually controlled. By using a third optical fiber (B), the absorbance of the bead layer can be monitored, allowing control of system performance.
This unique configuration, which allows simultaneous absorbance and fluorescence measurement in solutions or on the beads, may find a wide variety of novel applications.
Cell Configuration for
BI-Based Fluorescence
3.3.13.
